<HTML> <head> <title> Dispense 24-well hanging drop experiments with Oryx4 and Oryx8 </title> </head> <body lang=EN-US link="#3366CC" vlink="#3366CC" style='tab-interval:36.0pt;background-color:999999'> <div class=Section1> <div align=center> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:10pt 10pt 10pt 10pt'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'> <td style='padding:7.0pt 0cm 7.0pt 0cm;background:#cccccc'> <a href="http://www.douglas.co.uk/Newsletters/24HDNewsletter.html" target="_blank" style="text-decoration:none;text-underline:none"> <font size="2.0" color="white" face="arial" style='text-align:left'>&nbsp;&nbsp; View web version <font> </a> | <a href="http://www.douglas.co.uk" target="_blank" style="text-decoration:none"> <font size="2.0" color="white" face="arial" style='text-align:left'> www.douglas.co.uk <font> </a> </td> </tr> <tr style='mso-yfti-irow:1'> <td style='padding:0cm 0cm 0cm 0cm'> <img border=0 width=600 height=146" src="http://www.douglas.co.uk/images/HD24Banner.jpg" </td> </tr> <tr style='mso-yfti-irow:2'> <td style='padding:0cm 0cm 0cm 0cm'> <h1 align=center style='text-align:center'> <span style='mso-fareast-font-family:"Times New Roman";font-weight:normal'>&nbsp;</span> <span style='font-size:18.0pt;font-family:"Lucida Sans Unicode","sans-serif";mso-fareast-font-family: "Times New Roman";font-weight:normal'> Dispense 24-well hanging drop experiments with Oryx4 and Oryx8 </span> </h1> </td> </tr> <tr style='mso-yfti-irow:3'> <td width=600 style='width:450.0pt;padding:10.0pt 30.0pt 10pt 30.0pt'> <p class=MsoNormal> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> Douglas Instruments has released new software and hardware for automatic 24-well cover slide dispensing. The new method can dispense up to 5 drops per slide, making it easy to adjust the ratio of protein and reservoir solution in the drops, and to add e.g. seed stock or additive. <br><br> You can quickly set up powerful experiments that would take too long to do by hand. <br><br> The XStep optimization software also enables users to fill the reservoirs of the source plate. This makes creating PEG vs pH grids and multivariate optimization designs simple. The system can then dispense the drops. </span> </p> </td> </tr> <tr style='mso-yfti-irow:4'> <td style='padding:0cm 0cm 0cm 0cm'> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:0cm 0cm 0cm 0cm'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'> <td style='padding:0cm 0cm 0cm 0cm'> <p align=center style='margin-top:7.5pt;margin-right:0cm;margin-bottom: 7.5pt;margin-left:0cm;text-align:center'><strong> <span style='font-size: 11.0pt;font-family:"Arial","sans-serif";color:black'> 24-Well Hanging drop on Oryx4 and 8 </span></strong><span style='font-family:"Arial","sans-serif"; color:black'><o:p></o:p></span></p> <ul style='margin-top:0cm' type=disc> <p> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <strong> New hardware </strong> <br> 24-Well cover slide holder. </span><span style='font-family:"Arial","sans-serif"; mso-fareast-font-family:"Times New Roman"'><o:p></o:p></span> <p> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <strong> Use any 18 / 22mm cover slide </strong> <br> Compatible with both thick and thin cover slides. </span><span style='font-family:"Arial","sans-serif"; mso-fareast-font-family:"Times New Roman"'><o:p></o:p></span> <p> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <strong> Optimization </strong> <br> Use with our existing XStep software to create targeted screens or multivariate optimizations - in up to 7 dimensions! </span><span style='font-family:"Arial","sans-serif"; mso-fareast-font-family:"Times New Roman"'><o:p></o:p></span> <p> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <strong> Versatile </strong> <br> Oryx4 can dispense up to 3 ingredients per drop; Oryx8 can dispense up to 7 ingredients. </span><span style='font-family:"Arial","sans-serif"; mso-fareast-font-family:"Times New Roman"'><o:p></o:p></span> </ul> </td> <td style='padding:7.0pt 15.0pt 7.0pt 15.0pt'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'> <a href="https://www.youtube.com/watch?v=kcfcq695EDE" target="_blank"> <img border=0 width=250 height=200" src="http://www.douglas.co.uk/images/HD_main.jpg"> </a> <o:p></o:p></span></p> </td> </tr> </table> </td> </tr> <tr style='mso-yfti-irow:6'> <td style='padding:0cm 0cm 0cm 0cm'> <img border=0 width=600 height=1" src="http://www.douglas.co.uk/images/nl_border.jpg"> </td> </tr> <tr style='mso-yfti-irow:7'> <td style='padding:0cm 0cm 0cm 0cm'> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:0cm 0cm 0cm 0cm'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'> <td style='padding:0cm 0cm 0cm 30pt'> <p align=center style='margin-top:7.5pt;margin-right:0cm;margin-bottom: 7.5pt;margin-left:0cm;text-align:center'> <ul style='margin-top:0cm' type=disc> <a href="https://www.youtube.com/watch?v=kcfcq695EDE" target="_blank"> <img border=0 width=100 height=85" src="http://www.douglas.co.uk/images/Btn_24vid.jpg"> </a> <span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> </ul> </td> <td style='padding:7.0pt 15.0pt 7.0pt 15.0pt'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <strong> 24-Well Hanging Drop Video </strong> <br> Overview of the experiment on YouTube <br> <br> <a href="https://www.youtube.com/watch?v=kcfcq695EDE" target="_blank"> https://www.youtube.com/watch?v=kcfcq695EDE </a> </span><span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> <o:p></o:p></span></p> </td> </tr> </table> </td> </tr> <tr style='mso-yfti-irow:8'> <td style='padding:2pt 0cm 0cm 0cm'> <img border=0 width=600 height=1" src="http://www.douglas.co.uk/images/nl_border.jpg"> </td> </tr> <tr style='mso-yfti-irow:9'> <td style='padding:0cm 0cm 0cm 0cm'> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:0cm 0cm 0cm 0cm'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'> <td style='padding:0cm 0cm 0cm 30pt'> <p align=center style='margin-top:7.5pt;margin-right:0cm;margin-bottom: 7.5pt;margin-left:0cm;text-align:center'> <ul style='margin-top:0cm' type=disc> <a href="http://www.douglas.co.uk/oryx8.htm#Autodesign" target="_blank"> <img border=0 width=100 height=85" src="http://www.douglas.co.uk/images/nl_button7d.jpg"> </a> <span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> </ul> </td> <td style='padding:7.0pt 15.0pt 7.0pt 15.0pt'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <strong> Rapid reservoir filling for optimization with Oryx8 </strong> <br> Compatible with Linbro and VDX plates <br> <br> <a href="http://www.douglas.co.uk/oryx8.htm#Autodesign" target="_blank"> www.douglas.co.uk/oryx8.htm </a> </span><span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> <o:p></o:p></span></p> </td> </tr> </table> </td> </tr> <tr style='mso-yfti-irow:10'> <td style='padding:2pt 0cm 0cm 0cm'> <img border=0 width=600 height=1" src="http://www.douglas.co.uk/images/nl_border.jpg"> </td> </tr> <tr style='mso-yfti-irow:11'> <td style='padding:0cm 0cm 0cm 0cm'> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:0cm 0cm 0cm 0cm'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'> <td style='padding:0cm 0cm 0cm 30pt'> <p align=center style='margin-top:7.5pt;margin-right:0cm;margin-bottom: 7.5pt;margin-left:0cm;text-align:center'> <ul style='margin-top:0cm' type=disc> <a href="mailto:Anne-Marie@douglas.co.uk?subject=Quote request for 24 well cover slide holder"> <img border=0 width=100 height=85" src="http://www.douglas.co.uk/images/Btn_td24.jpg"> </a> <span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> </ul> </td> <td style='padding:7.0pt 15.0pt 7.0pt 15.0pt'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <strong> Order Hanging drop setup for Oryx4/8 </strong> <br> 24-Well cover slide holder. <br> Contact us for a quote or more information <br> <br> <a href="mailto:Anne-Marie@douglas.co.uk?subject=Quote request for 24 well cover slide holder"> Anne-Marie@douglas.co.uk </a> </span><span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> <o:p></o:p></span></p> </td> </tr> </table> </td> </tr> <tr style='mso-yfti-irow:12'> <td style='padding:2pt 0cm 0cm 0cm'> <img border=0 width=600 height=1" src="http://www.douglas.co.uk/images/nl_border.jpg"> </td> </tr> <tr style='mso-yfti-irow:13'> <td style='padding:10pt 0cm 0cm 0cm'> <p> </td> </tr> <tr style='mso-yfti-irow:14'> <td style='padding:0cm 0cm 0cm 0cm'> <h1 align=center style='text-align:center'> <span style='mso-fareast-font-family:"Times New Roman";font-weight:normal'>&nbsp;</span> <span style='font-size:12.0pt;font-family:"Lucida Sans Unicode","sans-serif";mso-fareast-font-family: "Times New Roman";font-weight:normal'> Technical tip: Scaling up from nano-drops to micro-drops </span> </h1> </td> </tr> <tr style='mso-yfti-irow:15'> <td width=600 style='width:450.0pt;padding:10.0pt 30.0pt 10pt 30.0pt'> <p class=MsoNormal> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> If you set up your initial screens with 100 nl (protein) + 100 nl (reservoir solution) drops, it's quite likely that you will have to scale up to larger drops in order to get crystals that are large enough to collect data from. <br><br> If you take the same solutions and simply increase the volume to say 1 + 1 l, you will often get precipitation instead of crystallization. This is a counterintuitive result because people correctly believe that the smaller drops will dry out more quickly, and they therefore assume that they will see more precipitation in the smaller drops. <br><br> However, the situation becomes clear when you realize that there are two important differences between large and small drops: <br><br> 1. The surface area to volume ratio is greater for the smaller drops (to put it another way, more of the small drop is close to the surface). <br><br> 2. The smaller drops equilibrate with the reservoir more quickly. <br><br> <a href="http://www.douglas.co.uk/Scaling_Up.htm" target="_blank"> <strong> Read more about Scaling up on our website& . </strong> </a> <br><br><br><br> </span> </p> </td> </tr> <tr style='mso-yfti-irow:16'> <td width=600 style='width:450.0pt;background:#cccccc;padding:1.0pt 35.0pt 3.0pt 35.0pt'> <p align=center style='text-align:center'><span style='font-size:10.0pt; font-family:"Arial","sans-serif";color:white'>Recent citations of Douglas Instruments products </span></p> </td> </tr> <tr style='mso-yfti-irow:17'> <td width=600 style='width:450.0pt;padding:10.0pt 30.0pt 10pt 30.0pt'> <p class=MsoNormal> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <a href="http://www.sciencedirect.com/science/article/pii/S0161589013001132" style="text-decoration:none;text-underline:none" target="_blank"> IgG2 Fc structure and the dynamic features of the IgG CH2 CH3 interface </a> <strong> <i> (using random Microseeding, rMMS) </strong> </i> <br> Alexey Teplyakov, Yonghong Zhao, Thomas J. Malia, Galina Obmolova, Gary L. Gilliland <br> Molecular Immunology, Volume 56,131 139 <br><br> <a href="http://www.jbc.org/content/288/8/5861.short" style="text-decoration:none;text-underline:none" target="_blank"> Structural, Biochemical, and Computational Characterization of the Glycoside Hydrolase Family 7 Cellobiohydrolase of the Tree-killing Fungus Heterobasidion irregulare </a> <br> Majid Haddad Momeni, Christina M. Payne, Henrik Hansson, Nils Egil Mikkelsen, Jesper Svedberg, ke Engstrm, Mats Sandgren, Gregg T. Beckham, and Jerry Sthlberg <br> Journal of Biological Chemistry, 288, 5861-5872 <br><br> <a href="http://www.jbc.org/content/288/25/18260.short" style="text-decoration:none;text-underline:none" target="_blank"> Structural and Biochemical Characterization of Compounds Inhibiting Mycobacterium tuberculosis Pantothenate Kinase </a> <br> Christofer Bjrkelid, Terese Bergfors, Anand Kumar V. Raichurkar, Kakoli Mukherjee, Krishnan Malolanarasimhan, Balachandra Bandodkar and T. Alwyn Jones <br> Journal of Biological Chemistry, 288, 18260-18270 <br><br> <a href="http://onlinelibrary.wiley.com/doi/10.1002/cbic.201300136/full" style="text-decoration:none;text-underline:none" target="_blank"> The Structure of Glycerol Trinitrate Reductase NerA from Agrobacterium radiobacter Reveals the Molecular Reason for Nitro- and Ene-Reductase Activity in OYE Homologues </a> <strong> <i> (using random Microseeding, rMMS) </strong> </i> <br> Dr. Gustav Oberdorfer, Dr. Alexandra Binter, Dr. Silvia Wallner, Dr. Katharina Durchschein, Dr. Mlanie Hall, Prof. Kurt Faber, Prof. Peter Macheroux, Prof. Karl Gruber <br> ChemBioChem, Volume 14, Issue 7, pages 836 845, May 10, 2013 <br><br> </span> </p> </td> </tr> <tr style='mso-yfti-irow:18'> <td width=600 style='width:450.0pt;background:#cccccc;padding:1.0pt 35.0pt 3.0pt 35.0pt'> <p align=center style='text-align:center'>&nbsp;</p> </td> </tr> <tr style='mso-yfti-irow:19'> <td width=600 style='width:450.0pt;background:white;padding:1.0pt 35.0pt 3.0pt 35.0pt'> <p align=center style='text-align:center'>&nbsp;</p> </td> </tr> <tr style='mso-yfti-irow:20'> <td width=600 style='width:450.0pt;padding:10.0pt 30.0pt 10pt 30.0pt'> <p class=MsoNormal> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> If you would like further information or have any questions please contact Stefan Kolek:<br><br> Tel: + (44) 1488 649090 US toll free: 1-877-225-2034 <br><br>or Stefan@douglas.co.uk <br><br> <br><br> </span> </p> </td> </tr> <tr style='mso-yfti-irow:21'> <td width=600 style='width:450.0pt;background:#D3D8FE;padding:1.0pt 35.0pt 3.0pt 35.0pt'> <p align=center style='text-align:center'><span style='font-size:10.0pt; font-family:"Arial","sans-serif";color:#505050'>To request a service please contact <a href="mailto:Stefan@douglas.co.uk" target="_blank">Stefan@douglas.co.uk</a>. <br> To request a demonstration please contact <a href="mailto:Hilary@douglas.co.uk" target="_blank">Hilary@douglas.co.uk</a> </span><span style='color:#505050'><o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:22'> <td style='padding:0cm 0cm 0cm 0cm'> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:0cm 0cm 0cm 0cm'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'> <td style='padding:10.0pt 22.0pt 10.0pt 22.0pt'> <p align=center style='text-align:center'><em><span style='font-size:10.0pt; font-family:"Arial","sans-serif";color:darkslategray'> Douglas Instruments Ltd. All rights reserved.</span></em><span style='color: darkslategray'><o:p></o:p></span></p> <p align=center style='text-align:center'> <span style='font-size:10.0pt;font-family:"Arial","sans-serif"; color:darkslategray'><br> Douglas Instruments Ltd <br> East Garston <br> Hungerford <br> Berkshire RG17 7HD <br> United Kingdom</span><span style='color:darkslategray'><o:p></o:p></span></p> <p align=center style='text-align:center'><span style='font-family:"Arial","sans-serif"; color:darkslategray'>Tel: + (44) 1488 649090 <br> US toll free: 1-877-225-2034 </span><span style='color:darkslategray'> </span></p> </td> <td width=280 style='width:210.0pt;padding:15.0pt 0cm 15.0pt 0cm'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'><img border=0 width=250 height=223 id="_x0000_i1029" src="http://www.douglas.co.uk/images/OryxRange.jpg" alt="The Oryx range" longdesc="The Oryx&#13;&#10;range of robots for automatic protein crystallization"><o:p></o:p></span></p> </td> </tr> </table> </td> </tr> <tr style='mso-yfti-irow:23'> <td style='padding:0cm 0cm 0cm 0cm'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'>&nbsp; &nbsp; <o:p></o:p></span></p> <div align=center> <table class=MsoNormalTable border=1 cellspacing=0 cellpadding=0 width=400 style='width:300.0pt;mso-cellspacing:0cm;background:#ECECEC;border:outset black 1.0pt; mso-border-alt:outset black .75pt;mso-yfti-tbllook:1184;mso-padding-alt: 3.75pt 3.75pt 3.75pt 3.75pt'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'> <td style='padding:4.5pt 3.75pt 3.75pt 3.75pt'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'><img border=0 width=132 height=26 id="_x0000_i1030" src="http://www.douglas.co.uk/images/GoogleGroups.gif" alt="Google Groups Logo"><o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:24'> <td style='padding:3.75pt 7.5pt 3.75pt 7.5pt'> <p><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'> Do you have a comment or question about protein crystallization or robotics that members of our bulletin board may be able to help you with? &nbsp; </span></p> <p><b><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'><a href="http://www.elabs5.com/c.html?rtr=on&amp;s=jazkd,dea8,uye,1z3w,6j7,doq8,32pd" target="_blank"> Subscribe</a> to the Automatic Protein Crystallization Group </span></b></p> </td> </tr> <tr style='mso-yfti-irow:25'> <td style='padding:3.75pt 3.75pt 3.75pt 3.75pt'></td> </tr> <tr style='mso-yfti-irow:26;mso-yfti-lastrow:yes'> <td style='padding:3.75pt 3.75pt 3.75pt 3.75pt'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'><a href="http://www.elabs5.com/c.html?rtr=on&amp;s=jazkd,dea8,uye,kzh2,ahkd,doq8,32pd" target="_blank">Visit this group</a><o:p></o:p></span></p> </td> </tr> </table> </div> </td> </tr> <tr style='mso-yfti-irow:27'> <td style='padding:10pt 0cm 0cm 0cm'> <p> </td> </tr> <tr style='mso-yfti-irow:28'> <td style='padding:0.0pt 0cm 0.0pt 0cm;background:#cccccc'> <h2 align=center style='text-align:center'><span style='font-size:12.0pt; font-family:"Lucida Sans Unicode","sans-serif";mso-fareast-font-family:"Times New Roman"; font-weight:normal;color:white'> Douglas Instruments </span><span style='mso-fareast-font-family: "Times New Roman";font-weight:normal'><o:p></o:p></span></h2> </td> </tr> </body> </html>