<HTML> <head> <title> Protein crystallization tips and tricks - analysis using polarizers with the UVXPO 'MRC' 2-drop plate </title> </head> <body lang=EN-US link="#3366CC" vlink="#3366CC" style='tab-interval:36.0pt;background-color:999999'> <div class=Section1> <div align=center> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:10pt 10pt 10pt 10pt'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'> <td style='padding:7.0pt 0cm 7.0pt 0cm;background:#cccccc'> <a href="http://www.douglas.co.uk/Newsletters/Feb_2016_Newsletter.html" target="_blank" style="text-decoration:none;text-underline:none"> <font size="2.0" color="white" face="arial" style='text-align:left'>&nbsp;&nbsp; View web version <font> </a> | <a href="http://www.douglas.co.uk" target="_blank" style="text-decoration:none"> <font size="2.0" color="white" face="arial" style='text-align:left'> www.douglas.co.uk <font> </a> </td> </tr> <tr style='mso-yfti-irow:2'> <td style='padding:0cm 0cm 0cm 0cm'> <h1 align=center style='text-align:center'> <span style='font-size:16.0pt;font-family:"Arial","sans-serif";color:black'> <br> <strong> Protein crystallization tips and tricks - analysis using polarizers with the UVXPO 'MRC' 2-drop plate </strong> </span> </h1> </td> </tr> <tr> <td width=600 style='width:450.0pt;padding:10.0pt 30.0pt 10pt 30.0pt'> <p class=MsoNormal> <span style='font-size: 11.0pt;font-family:"Arial","sans-serif";color:black'> The new <a href="http://www.douglas.co.uk/products.htm#DICONSUMABLES" target="_blank">UVXPO 'MRC' 2 drop plate</a> from Swissci offers advances in UV and cross-polarized imaging. The new plate has exactly the same dimensions as the original MRC 2-drop crystallization plate, but is made from a special polymer that is designed to maximize UV and polarized light. <br><br> Key features of the UVXPO 'MRC' 2-drop plate: <br><br> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-&nbsp;&nbsp;Uniform background when using polarized light. <br> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-&nbsp;&nbsp;UV imager compatible. <br> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-&nbsp;&nbsp;More hydrophilic surface than the original MRC 2 drop UV plate (COC) gives better dispensing. <br> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-&nbsp;&nbsp;<a href="http://www.douglas.co.uk/products.htm" target="_blank">OryxNano, Oryx4 and Oryx8</a> can dispense screening and optimization experiments. <br> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-&nbsp;&nbsp;<a href="http://www.douglas.co.uk/oryx8.htm#RapidResFill" target="_blank">Oryx8</a> can dispense reservoir solutions for optimization. <br> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-&nbsp;&nbsp;<a href="http://www.douglas.co.uk/oryx8.htm#LCPdisp" target="_blank">Oryx LCP systems</a> can dispense LCP experiments to the plate. <br><br> The UVXPO 2-drop plate is <a href="http://www.douglas.co.uk/products.htm#DICONSUMABLES" target="_blank">available from Douglas Instruments</a>. <br> <br><br> <strong> Tips and tricks for using the UVXPO plate: </strong> <br> <br> Although the UVXPO MRC plate has a very low uniform birefringence, when the plate is sealed, often the seal does have birefringence and acts a wave plate. This means one is unable to get a true black or white background as the polarizer is rotated with a normal plate seal. <br><br> To cancel out the waveplate effect, simply add a second layer of the same plate seal exactly perpendicular to the first.<br><br> <br><br> <center> <a href="http://www.douglas.co.uk/images/Newsletters/uvxpo_grid.png" target="_blank"> <img border=0 width=680 src="http://www.douglas.co.uk/images/Newsletters/uvxpo_grid.png"> </a> <br> <br><br> <center> <a href="http://www.douglas.co.uk/images/Newsletters/uvxpo_seal.png" target="_blank"> <img width=480 src="http://www.douglas.co.uk/images/Newsletters/uvxpo_seal.png"> </a> </center> </span> </p> </td> </tr> <tr style='mso-yfti-irow:19'> <td width=600 style='width:450.0pt;background:white;padding:1.0pt 35.0pt 3.0pt 35.0pt'> <p align=center style='text-align:center'>&nbsp;</p> </td> </tr> <tr style='mso-yfti-irow:6'> <td style='padding:0cm 0cm 0cm 0cm'> <img border=0 width=800 height=1" src="http://www.douglas.co.uk/images/nl_border.jpg"> </td> </tr> <tr style='mso-yfti-irow:21'> <td width=600 style='width:450.0pt;background:#D3D8FE;padding:1.0pt 35.0pt 3.0pt 35.0pt'> <p align=center style='text-align:center'><span style='font-size:10.0pt; font-family:"Arial","sans-serif";color:#505050'>To request a quote or demonstration please contact <a href="mailto:Hilary@douglas.co.uk" target="_blank">Hilary@douglas.co.uk</a>. <br> <br> To request product support contact <a href="mailto:Stefan@douglas.co.uk" target="_blank">Stefan@douglas.co.uk</a> <br> <br> For anything else please contact <a href="mailto:Info@douglas.co.uk" target="_blank">Info@douglas.co.uk</a> </span><span style='color:#505050'><o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:6'> <td style='padding:0cm 0cm 0cm 0cm'> <img border=0 width=800 height=1" src="http://www.douglas.co.uk/images/nl_border.jpg"> </td> </tr> <tr style='mso-yfti-irow:2'> <td style='padding:0cm 0cm 0cm 0cm'> <h1 align=center style='text-align:center'> <span style='font-size:16.0pt;font-family:"Arial","sans-serif";color:black'> <br> Douglas Instruments will be at the following meetings: </span> </h1> </td> </tr> <tr> <td width=600 style='width:450.0pt;padding:10.0pt 30.0pt 10pt 30.0pt'> <p class=MsoNormal> <span style='font-size: 11.0pt;font-family:"Arial","sans-serif";color:black'> Visit our booth and pick up a microseeding toolkit containing everything you need to do a <a href="http://www.douglas.co.uk/resrep.htm" target="_blank"><strong> MMS Microseeding experiment </strong></a> including the Hampton seed bead and crystal crusher. </span> </p> </td> </tr> <tr style='mso-yfti-irow:6'> <td style='padding:0cm 0cm 0cm 0cm'> <tr style='mso-yfti-irow:6'> <td style='padding:0cm 0cm 0cm 0cm'> <img border=0 width=800 height=1" src="http://www.douglas.co.uk/images/nl_border.jpg"> </td> </tr> <tr style='mso-yfti-irow:7'> <td style='padding:0cm 0cm 0cm 0cm'> <table class=MsoNormalTable border=0 cellspacing=0 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style='font-size:11.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <a href="http://www.eie.gr/nhrf/institutes/ibmcb/News/2016/14_02_2016_Workshop.html" target="_blank"><strong> Dilemmas in structural biology, NHRF, Athens </strong></a> </span> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <br><br> 14  17 February 2016 <br> </span><span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> <o:p></o:p></span></p> </td> </tr> </table> </td> </tr> <tr style='mso-yfti-irow:6'> <td style='padding:0cm 0cm 0cm 0cm'> <img border=0 width=800 height=1" src="http://www.douglas.co.uk/images/nl_border.jpg"> </td> </tr> <tr style='mso-yfti-irow:7'> <td style='padding:0cm 0cm 0cm 0cm'> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:0cm 0cm 0cm 0cm'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'> <td style='padding:0cm 0cm 0cm 15pt'> <p align=center style='margin-top:7.5pt;margin-right:0cm;margin-bottom: 7.5pt;margin-left:0cm;text-align:center'> <ul style='margin-top:0cm' type=disc> <a href="http://bca2016.crystallography.org.uk/" target="_blank"> <img border=0 width=135 src="http://www.douglas.co.uk/images/Newsletters/BCA2016.png" style="padding:1px;border:thin solid black;"> </a> <span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> </ul> </td> <td style='padding:7.0pt 15.0pt 7.0pt 15.0pt'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'> <span style='font-size:11.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <a href="http://bca2016.crystallography.org.uk/" target="_blank"><strong> BCA 2016 Nottingham </strong></a> </span> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <br><br> April 4th - 7th. <br> </span><span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> <o:p></o:p></span></p> </td> </tr> </table> </td> </tr> <tr style='mso-yfti-irow:6'> <td style='padding:0cm 0cm 0cm 0cm'> <img border=0 width=800 height=1" src="http://www.douglas.co.uk/images/nl_border.jpg"> </td> </tr> <tr style='mso-yfti-irow:7'> <td style='padding:0cm 0cm 0cm 0cm'> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:0cm 0cm 0cm 0cm'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'> <td style='padding:0cm 0cm 0cm 15pt'> <p align=center style='margin-top:7.5pt;margin-right:0cm;margin-bottom: 7.5pt;margin-left:0cm;text-align:center'> <ul style='margin-top:0cm' type=disc> <a href="http://smb.slac.stanford.edu/news/cryst_focus_on/cryst_focus_on_2016/index.html" target="_blank"> <img border=0 width=135 src="http://www.douglas.co.uk/images/Newsletters/slac.png" style="padding:1px;border:thin solid black;"> </a> <span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> </ul> </td> <td style='padding:7.0pt 15.0pt 7.0pt 15.0pt'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'> <span style='font-size:11.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <a href="http://smb.slac.stanford.edu/news/cryst_focus_on/cryst_focus_on_2016/index.html" target="_blank"><strong> Crystallization: focus on micro and nano crystals and high throughput methods, 2016 at SLAC </strong></a> </span> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <br><br> April 19th - 22nd. <br> </span><span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> <o:p></o:p></span></p> </td> </tr> </table> </td> </tr> <tr style='mso-yfti-irow:6'> <td style='padding:0cm 0cm 0cm 0cm'> <img border=0 width=800 height=1" src="http://www.douglas.co.uk/images/nl_border.jpg"> </td> </tr> <tr style='mso-yfti-irow:7'> <td style='padding:0cm 0cm 0cm 0cm'> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:0cm 0cm 0cm 0cm'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'> <td style='padding:0cm 0cm 0cm 15pt'> <p align=center style='margin-top:7.5pt;margin-right:0cm;margin-bottom: 7.5pt;margin-left:0cm;text-align:center'> <ul style='margin-top:0cm' type=disc> <a href="http://febs.img.cas.cz/index.php" target="_blank"> <img border=0 width=135 src="http://www.douglas.co.uk/images/Newsletters/FEBS2016.png" style="padding:1px;border:thin solid black;"> </a> <span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> </ul> </td> <td style='padding:7.0pt 15.0pt 7.0pt 15.0pt'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'> <span style='font-size:11.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <a href="http://febs.img.cas.cz/index.php" target="_blank"><strong> FEBS Advanced methods in macromolecular crystallization, Nove Hrady </strong></a> </span> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <br><br> June 27th - July 2nd. <br> </span><span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> <o:p></o:p></span></p> </td> </tr> </table> </td> </tr> <tr style='mso-yfti-irow:6'> <td style='padding:0cm 0cm 0cm 0cm'> <img border=0 width=800 height=1" src="http://www.douglas.co.uk/images/nl_border.jpg"> </td> </tr> <tr style='mso-yfti-irow:7'> <td style='padding:0cm 0cm 0cm 0cm'> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:0cm 0cm 0cm 0cm'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'> <td style='padding:0cm 0cm 0cm 15pt'> <p align=center style='margin-top:7.5pt;margin-right:0cm;margin-bottom: 7.5pt;margin-left:0cm;text-align:center'> <ul style='margin-top:0cm' type=disc> <a href="http://www.iccbm16.org/" target="_blank"> <img border=0 width=135 src="http://www.douglas.co.uk/images/Newsletters/ICCBM2016.png" style="padding:1px;border:thin solid black;"> </a> <span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> </ul> </td> <td style='padding:7.0pt 15.0pt 7.0pt 15.0pt'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'> <span style='font-size:11.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <a href="http://www.iccbm16.org/" target="_blank"><strong> ICCBM 2016 Prague </strong></a> </span> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <br><br> July 2nd - 7th. <br> </span><span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> <o:p></o:p></span></p> </td> </tr> </table> </td> </tr> <tr style='mso-yfti-irow:6'> <td style='padding:0cm 0cm 0cm 0cm'> <img border=0 width=800 height=1" src="http://www.douglas.co.uk/images/nl_border.jpg"> </td> </tr> <tr style='mso-yfti-irow:13'> <td style='padding:10pt 0cm 0cm 0cm'> <p> </td> </tr> <tr style='mso-yfti-irow:16'> <td width=600 style='width:450.0pt;background:#cccccc;padding:1.0pt 35.0pt 3.0pt 35.0pt'> <p align=center style='text-align:center'><span style='font-size:10.0pt; font-family:"Arial","sans-serif";color:white'>Recent citations of Douglas Instruments products </span></p> </td> </tr> <tr style='mso-yfti-irow:17'> <td width=600 style='width:450.0pt;padding:10.0pt 30.0pt 10pt 30.0pt'> <p class=MsoNormal> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <a href="http://onlinelibrary.wiley.com/doi/10.1002/prot.24988/full" style="text-decoration:none;text-underline:none" target="_blank"> Epitope mapping and structural basis for the recognition of phosphorylated tau by the anti tau antibody AT8 </a> <br><br> Malia, T.J., Teplyakov, A., Ernst, R., Wu, S.J., Lacy, E.R., Liu, X., Vandermeeren, M., Mercken, M., Luo, J., Sweet, R.W. and Gilliland, G.L. <br><br> Proteins. doi:10.1002/prot.24988 (2016) <br><br><br> <a href="http://link.springer.com/protocol/10.1007/978-1-4939-2871-2_20" style="text-decoration:none;text-underline:none" target="_blank"> Purification and Bicelle Crystallization for Structure Determination of the E. coli Outer Membrane Protein TamA </a> <br><br> Fabian Gruss, Sebastian Hiller, Timm Maier <br><br> Methods in Molecular Biology 1329 (2014): 259-270 <br><br><br> <a href="http://scripts.iucr.org/cgi-bin/paper?dw5137" style="text-decoration:none;text-underline:none" target="_blank"> Structure of the novel monomeric glyoxalase I from Zea mays </a> <br><br> G. L. Turra, R. B. Agostini, C. M. Fauguel, D. A. Presello, C. S. Andreo, J. M. Gonzlez and V. A. Campos-Bermudez <br><br> Acta Cryst D 71.10 (2015): 2009-2020 <br><br><br> <a href="http://www.nature.com/articles/srep15337?trendmd-shared=0" style="text-decoration:none;text-underline:none" target="_blank"> Discovery and characterization of natural tropolones as inhibitors of the antibacterial target CapF from Staphylococcus aureus </a> <br><br> Koichiro Nakano, Takeru Chigira, Takamitsu Miyafusa, Satoru Nagatoishi, Jose M. M. Caaveiro & Kouhei Tsumoto <br><br> Scientific Reports 5 (2015): 15337 <br> </span> </p> </td> </tr> <tr style='mso-yfti-irow:18'> <td width=600 style='width:450.0pt;background:#cccccc;padding:1.0pt 35.0pt 3.0pt 35.0pt'> <p align=center style='text-align:center'>&nbsp;</p> </td> </tr> <tr style='mso-yfti-irow:19'> <td width=600 style='width:450.0pt;background:white;padding:1.0pt 35.0pt 3.0pt 35.0pt'> <p align=center style='text-align:center'>&nbsp;</p> </td> </tr> <tr style='mso-yfti-irow:22'> <td style='padding:0cm 0cm 0cm 0cm'> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:0cm 0cm 0cm 0cm'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'> <td style='padding:10.0pt 22.0pt 10.0pt 22.0pt'> <p align=center style='text-align:center'><em><span style='font-size:10.0pt; font-family:"Arial","sans-serif";color:darkslategray'> Douglas Instruments Ltd. 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