<HTML> <head> <title> Optimization with Oryx8 </title> </head> <body lang=EN-US link="#3366CC" vlink="#3366CC" style='tab-interval:36.0pt;background-color:999999'> <div class=Section1> <div align=center> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:10pt 10pt 10pt 10pt'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'> <td style='padding:7.0pt 0cm 7.0pt 0cm;background:#cccccc'> <a href="http://www.douglas.co.uk/Newsletters/24HDNewsletter.html" target="_blank" style="text-decoration:none;text-underline:none"> <font size="2.0" color="white" face="arial" style='text-align:left'>&nbsp;&nbsp; View web version <font> </a> | <a href="http://www.douglas.co.uk" target="_blank" style="text-decoration:none"> <font size="2.0" color="white" face="arial" style='text-align:left'> www.douglas.co.uk <font> </a> </td> </tr> <tr style='mso-yfti-irow:1'> <td style='padding:0cm 0cm 0cm 0cm'> <img border=0 width=600 height=244 src="http://www.douglas.co.uk/images/NL_Opt_Banner.jpg"> </td> </tr> <tr style='mso-yfti-irow:2'> <td style='padding:0cm 0cm 0cm 0cm'> <h1 align=center style='text-align:center'> <span style='mso-fareast-font-family:"Times New Roman";font-weight:normal'>&nbsp;</span> <span style='font-size:18.0pt;font-family:"Lucida Sans Unicode","sans-serif";mso-fareast-font-family: "Times New Roman";font-weight:normal'> <strong> Optimization with Oryx8 </strong> </span> </h1> </td> </tr> <tr style='mso-yfti-irow:3'> <td width=600 style='width:450.0pt;padding:10.0pt 30.0pt 10pt 30.0pt'> <p class=MsoNormal> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> Optimization of a hit condition is not usually a straight forward procedure. Sometimes it may be necessary to add an ingredient such as seed or additive to the drop; equally it may be necessary to reduce reservoir concentration or remove an ingredient. Choice of a suitable experimental method , whether Under-Oil, Sitting Drop, Hanging Drop or LCP, also needs careful consideration. <br><br> Douglas Instruments has recently released a major update for optimization with Oryx8. This enables users to create optimization designs for Sitting Drop, Hanging Drop and Microbatch-under-oil. Our optimization software, Xstep, allows users to design powerful optimization experiments with up to 7 ingredients. The software has tools for gradient designs, multivariate designs and pH experiments, and introduces a new reservoir-dispensing capability. <br><br> When clicking dispense the user is asked to place the required solutions onto the robot work table. The robot then pipettes reservoir solutions, one at a time, using a disposable 1 mL tip. Typically, a 96-well 6 ingredient multivariate design with a 40 L reservoir takes less than 20 minutes to dispense. <br><br> Oryx8 then uses the Microtip to dispense drops with protein (also where specified, reservoir solutions that require very accurate dispensing) along with buffer and other additives. </span> </p> </td> </tr> <tr style='mso-yfti-irow:4'> <td width=600 style='width:450.0pt;padding:10.0pt 30.0pt 10pt 30.0pt'> <p class=MsoNormal> <center> <img border=0 width=400 height=212 src="http://www.douglas.co.uk/images/NLOptPlates.jpg"> </center> </p> </td> </tr> <tr style='mso-yfti-irow:4'> <td style='padding:0cm 0cm 0cm 0cm'> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:0cm 0cm 0cm 0cm'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'> <td style='padding:0cm 0cm 0cm 0cm'> <p align=center style='margin-top:7.5pt;margin-right:0cm;margin-bottom: 7.5pt;margin-left:0cm;text-align:center'><strong> <span style='font-size: 11.0pt;font-family:"Arial","sans-serif";color:black'> Optimization with Oryx8 </span></strong><span style='font-family:"Arial","sans-serif"; color:black'><o:p></o:p></span></p> <ul style='margin-top:0cm' type=disc> <p> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <strong> Different types of optimization </strong> <br> Vapour Diffusion, Microbatch Under-Oil or Hanging Drop.. </span><span style='font-family:"Arial","sans-serif"; mso-fareast-font-family:"Times New Roman"'><o:p></o:p></span> <p> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <strong> Create optimization stock plates </strong> <br> Dispense custom experiments to any plate including SBS or Linbro format plates. </span><span style='font-family:"Arial","sans-serif"; mso-fareast-font-family:"Times New Roman"'><o:p></o:p></span> <p> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <strong> Easy and Powerful </strong> <br> Design your experiment and click dispense! </span><span style='font-family:"Arial","sans-serif"; mso-fareast-font-family:"Times New Roman"'><o:p></o:p></span> <p> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <strong> Versatile </strong> <br> Dispense drops from 100 + 100 nL to 4 + 4 L. Dispense reservoirs from 20 L upto 600 L. </span><span style='font-family:"Arial","sans-serif"; mso-fareast-font-family:"Times New Roman"'><o:p></o:p></span> </ul> </td> <td style='padding:7.0pt 15.0pt 7.0pt 15.0pt'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'> <a href="http://www.youtube.com/watch?v=0WvR3bHzgi8" target="_blank"> <img border=0 width=250 height=168 src="http://www.douglas.co.uk/images/NL_Opt_PlateMain.jpg"> </a> <o:p></o:p></span></p> </td> </tr> </table> </td> </tr> <tr style='mso-yfti-irow:6'> <td style='padding:0cm 0cm 0cm 0cm'> <img border=0 width=600 height=1 src="http://www.douglas.co.uk/images/nl_border.jpg"> </td> </tr> <tr style='mso-yfti-irow:7'> <td style='padding:0cm 0cm 0cm 0cm'> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:0cm 0cm 0cm 0cm'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'> <td style='padding:0cm 0cm 0cm 30pt'> <p align=center style='margin-top:7.5pt;margin-right:0cm;margin-bottom: 7.5pt;margin-left:0cm;text-align:center'> <ul style='margin-top:0cm' type=disc> <a href="http://www.youtube.com/watch?v=0WvR3bHzgi8" target="_blank"> <img border=0 width=100 height=85 src="http://www.douglas.co.uk/images/NL_BTN_VID.jpg"> </a> <span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> </ul> </td> <td style='padding:7.0pt 15.0pt 7.0pt 15.0pt'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <strong> Video of optimization experiment </strong> <br> Overview of an experiment on YouTube <br> <br> <a href="http://www.youtube.com/watch?v=0WvR3bHzgi8" target="_blank"> http://www.youtube.com/watch?v=0WvR3bHzgi8 </a> </span><span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> <o:p></o:p></span></p> </td> </tr> </table> </td> </tr> <tr style='mso-yfti-irow:8'> <td style='padding:2pt 0cm 0cm 0cm'> <img border=0 width=600 height=1 src="http://www.douglas.co.uk/images/nl_border.jpg"> </td> </tr> <tr style='mso-yfti-irow:9'> <td style='padding:0cm 0cm 0cm 0cm'> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:0cm 0cm 0cm 0cm'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'> <td style='padding:0cm 0cm 0cm 30pt'> <p align=center style='margin-top:7.5pt;margin-right:0cm;margin-bottom: 7.5pt;margin-left:0cm;text-align:center'> <ul style='margin-top:0cm' type=disc> <a href="http://www.douglas.co.uk/oryx8.htm" target="_blank"> <img border=0 width=100 height=85 src="http://www.douglas.co.uk/images/nl_button7d.jpg"> </a> <span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> </ul> </td> <td style='padding:7.0pt 15.0pt 7.0pt 15.0pt'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <strong> Rapid reservoir filling for optimization with Oryx8 </strong> <br> Oryx8 on our website <br> <br> <a href="http://www.douglas.co.uk/oryx8.htm" target="_blank"> www.douglas.co.uk/oryx8.htm </a> </span><span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> <o:p></o:p></span></p> </td> </tr> </table> </td> </tr> <tr style='mso-yfti-irow:10'> <td style='padding:2pt 0cm 0cm 0cm'> <img border=0 width=600 height=1 src="http://www.douglas.co.uk/images/nl_border.jpg"> </td> </tr> <tr style='mso-yfti-irow:11'> <td style='padding:0cm 0cm 0cm 0cm'> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:0cm 0cm 0cm 0cm'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'> <td style='padding:0cm 0cm 0cm 30pt'> <p align=center style='margin-top:7.5pt;margin-right:0cm;margin-bottom: 7.5pt;margin-left:0cm;text-align:center'> <ul style='margin-top:0cm' type=disc> <a href="http://www.douglas.co.uk/f_ftp1/XStep_Quick_Start.pdf"> <img border=0 width=100 height=85 src="http://www.douglas.co.uk/images/NL_BTN_PDF.jpg"> </a> <span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> </ul> </td> <td style='padding:7.0pt 15.0pt 7.0pt 15.0pt'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <strong> User manual for designing experiments </strong> <br> Step-by-step instructions for designing experiments with Xstep <br> <br> <br> <a href="http://www.douglas.co.uk/f_ftp1/XStep_Quick_Start.pdf" target="_blank"> XStep_Quick_Start.pdf </a> </span><span style='font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'><o:p></o:p></span> <o:p></o:p></span></p> </td> </tr> </table> </td> </tr> <tr style='mso-yfti-irow:12'> <td style='padding:2pt 0cm 0cm 0cm'> <img border=0 width=600 height=1 src="http://www.douglas.co.uk/images/nl_border.jpg"> </td> </tr> <tr style='mso-yfti-irow:13'> <td style='padding:10pt 0cm 0cm 0cm'> <p> </td> </tr> <tr style='mso-yfti-irow:14'> <td style='padding:0cm 0cm 0cm 0cm'> <h1 align=center style='text-align:center'> <span style='mso-fareast-font-family:"Times New Roman";font-weight:normal'>&nbsp;</span> <span style='font-size:12.0pt;font-family:"Lucida Sans Unicode","sans-serif";mso-fareast-font-family: "Times New Roman";font-weight:normal'> Technical tip: Using a  Combinatorial Optimization script to optimize Random Microseeding experiments </span> </h1> </td> </tr> <tr style='mso-yfti-irow:15'> <td width=600 style='width:450.0pt;padding:10.0pt 30.0pt 10pt 30.0pt'> <p class=MsoNormal> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> A typical scenario after doing a random microseeding experiment is that there are too many crystals or nucleation sites in the drop, and it is not obvious where to begin optimization. (Typically random microseeding experiments require a very concentrated seed stock to maximize the chance of crystal growth; however this can lead to over nucleation and thousands of tiny micro crystals!) by creating a dilution series it is possible to systematically identify the correct dilution of seed stock for the protein and crystallization condition. <br><br> Douglas Instruments "Combinatorial Optimization" script allows users to trial up to 12 different seed solutions (or additives) and up to 8 different precipitants with the same protein. Known conditions for crystal growth can be quickly optimized. This is a great way to control the extent of crystal growth and get the right number of crystals. <br> <center> <img border=0 width=200 height=227 src="http://www.douglas.co.uk/images/NL_Combinatorial_2.jpg"> </center> <br> <a href="http://www.douglas.co.uk/combinatorial.htm" target="_blank"> <strong> To read more about the  combinatorial optimization click here </strong> </a> <br><br><br><br> </span> </p> </td> </tr> <tr style='mso-yfti-irow:16'> <td width=600 style='width:450.0pt;background:#cccccc;padding:1.0pt 35.0pt 3.0pt 35.0pt'> <p align=center style='text-align:center'><span style='font-size:10.0pt; font-family:"Arial","sans-serif";color:white'>Recent citations of Douglas Instruments products </span></p> </td> </tr> <tr style='mso-yfti-irow:17'> <td width=600 style='width:450.0pt;padding:10.0pt 30.0pt 10pt 30.0pt'> <p class=MsoNormal> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> <a href="http://scripts.iucr.org/cgi-bin/paper_yard?wd5214" style="text-decoration:none;text-underline:none" target="_blank"> Structure of arylamine N-acetyltransferase from Mycobacterium tuberculosis determined by cross-seeding with the homologous protein from M. marinum: triumph over adversity </a> <strong> <i><br> (Random Microseeding, rMMS) </strong> </i> <br> A. Abuhammad, E. D. Lowe, M. A. McDonough, <strong><u><i> P. D. Shaw Stewart</i></u></strong>, <strong><u><i>S. A. Kolek</i></u></strong>, E. Sim and E. F. Garman <br> Acta Cryst. (2013). D69, 1433-1446 <br><br> <a href="http://scripts.iucr.org/cgi-bin/paper?S1744309113019714" style="text-decoration:none;text-underline:none" target="_blank"> The binding of zinc ions to Emericella nidulans endo-[beta]-1,4-galactanase is essential for crystal formation </a> <br> H. Otten, M. Michalak, J. D. Mikkelsen and S. Larsen <br> Acta Cryst. (2013). F69, 850-854 <br><br> <a href="http://scripts.iucr.org/cgi-bin/paper?gm5022" style="text-decoration:none;text-underline:none" target="_blank"> Protein HP1028 from the human pathogen Helicobacter pylori belongs to the lipocalin family </a> <br> N. Barison, L. Cendron, V. Loconte, E. A. Proctor, N. V. Dokholyan and G. Zanotti <br> Acta Cryst. (2013). D69, 1387-1394 <br><br> <a href="http://www.sciencedirect.com/science/article/pii/S0005273613002447" style="text-decoration:none;text-underline:none" target="_blank"> Membrane protein structure determination  The next generation </a> <strong> <i><br> (Random Microseeding, rMMS with membrane proteins) </strong> </i> <br> Isabel Moraes, Gwyndaf Evans, Juan Sanchez-Weatherby, Simon Newstead, <strong><u><i> Patrick D. Shaw Stewart </i></u></strong> <br> j.bbamem.2013.07.010 <br><br> </span> </p> </td> </tr> <tr style='mso-yfti-irow:18'> <td width=600 style='width:450.0pt;background:#cccccc;padding:1.0pt 35.0pt 3.0pt 35.0pt'> <p align=center style='text-align:center'>&nbsp;</p> </td> </tr> <tr style='mso-yfti-irow:19'> <td width=600 style='width:450.0pt;background:white;padding:1.0pt 35.0pt 3.0pt 35.0pt'> <p align=center style='text-align:center'>&nbsp;</p> </td> </tr> <tr style='mso-yfti-irow:20'> <td width=600 style='width:450.0pt;padding:10.0pt 30.0pt 10pt 30.0pt'> <p class=MsoNormal> <span style='font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family: "Times New Roman"'> If you would like further information or have any questions please contact Stefan Kolek:<br><br> Tel: + (44) 1488 649090 US toll free: 1-877-225-2034 <br><br>or Stefan@douglas.co.uk <br><br> <br><br> </span> </p> </td> </tr> <tr style='mso-yfti-irow:21'> <td width=600 style='width:450.0pt;background:#D3D8FE;padding:1.0pt 35.0pt 3.0pt 35.0pt'> <p align=center style='text-align:center'><span style='font-size:10.0pt; font-family:"Arial","sans-serif";color:#505050'>To request a service please contact <a href="mailto:Stefan@douglas.co.uk" target="_blank">Stefan@douglas.co.uk</a>. <br> To request a demonstration please contact <a href="mailto:Hilary@douglas.co.uk" target="_blank">Hilary@douglas.co.uk</a> </span><span style='color:#505050'><o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:22'> <td style='padding:0cm 0cm 0cm 0cm'> <table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0 width=600 style='width:450.0pt;mso-cellspacing:0cm;background:white;mso-yfti-tbllook: 1184;mso-padding-alt:0cm 0cm 0cm 0cm'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'> <td style='padding:10.0pt 22.0pt 10.0pt 22.0pt'> <p align=center style='text-align:center'><em><span style='font-size:10.0pt; font-family:"Arial","sans-serif";color:darkslategray'> Douglas Instruments Ltd. All rights reserved.</span></em><span style='color: darkslategray'><o:p></o:p></span></p> <p align=center style='text-align:center'> <span style='font-size:10.0pt;font-family:"Arial","sans-serif"; color:darkslategray'><br> Douglas Instruments Ltd <br> East Garston <br> Hungerford <br> Berkshire RG17 7HD <br> United Kingdom</span><span style='color:darkslategray'><o:p></o:p></span></p> <p align=center style='text-align:center'><span style='font-family:"Arial","sans-serif"; color:darkslategray'>Tel: + (44) 1488 649090 <br> US toll free: 1-877-225-2034 </span><span style='color:darkslategray'> </span></p> </td> <td width=280 style='width:210.0pt;padding:15.0pt 0cm 15.0pt 0cm'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'><img border=0 width=250 height=223 id="_x0000_i1029" src="http://www.douglas.co.uk/images/OryxRange.jpg" alt="The Oryx range" longdesc="The Oryx&#13;&#10;range of robots for automatic protein crystallization"><o:p></o:p></span></p> </td> </tr> </table> </td> </tr> <tr style='mso-yfti-irow:23'> <td style='padding:0cm 0cm 0cm 0cm'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'>&nbsp; &nbsp; <o:p></o:p></span></p> <div align=center> <table class=MsoNormalTable border=1 cellspacing=0 cellpadding=0 width=400 style='width:300.0pt;mso-cellspacing:0cm;background:#ECECEC;border:outset black 1.0pt; mso-border-alt:outset black .75pt;mso-yfti-tbllook:1184;mso-padding-alt: 3.75pt 3.75pt 3.75pt 3.75pt'> <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'> <td style='padding:4.5pt 3.75pt 3.75pt 3.75pt'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'><img border=0 width=132 height=26 id="_x0000_i1030" src="http://www.douglas.co.uk/images/GoogleGroups.gif" alt="Google Groups Logo"><o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:24'> <td style='padding:3.75pt 7.5pt 3.75pt 7.5pt'> <p><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'> Do you have a comment or question about protein crystallization or robotics that members of our bulletin board may be able to help you with? &nbsp; </span></p> <p><b><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'><a href="http://www.elabs5.com/c.html?rtr=on&amp;s=jazkd,dea8,uye,1z3w,6j7,doq8,32pd" target="_blank"> Subscribe</a> to the Automatic Protein Crystallization Group </span></b></p> </td> </tr> <tr style='mso-yfti-irow:25'> <td style='padding:3.75pt 3.75pt 3.75pt 3.75pt'></td> </tr> <tr style='mso-yfti-irow:26;mso-yfti-lastrow:yes'> <td style='padding:3.75pt 3.75pt 3.75pt 3.75pt'> <p class=MsoNormal align=center style='text-align:center'><span style='mso-fareast-font-family:"Times New Roman"'><a href="http://www.elabs5.com/c.html?rtr=on&amp;s=jazkd,dea8,uye,kzh2,ahkd,doq8,32pd" target="_blank">Visit this group</a><o:p></o:p></span></p> </td> </tr> </table> </div> </td> </tr> <tr style='mso-yfti-irow:27'> <td style='padding:10pt 0cm 0cm 0cm'> <p> </td> </tr> <tr style='mso-yfti-irow:28'> <td style='padding:0.0pt 0cm 0.0pt 0cm;background:#cccccc'> <h2 align=center style='text-align:center'><span style='font-size:12.0pt; font-family:"Lucida Sans Unicode","sans-serif";mso-fareast-font-family:"Times New Roman"; font-weight:normal;color:white'> Douglas Instruments </span><span style='mso-fareast-font-family: "Times New Roman";font-weight:normal'><o:p></o:p></span></h2> </td> </tr> </body> </html>