Oryx8 is the flagship Douglas Instruments protein crystallization robot. It can set up sitting drops down to 100 + 100 nl and up to 2 + 2 µl for 96 wells. For hanging drop experiments up to five drops can be set up per cover slide, with volumes up to 8 + 8 µl. (For screening experiments the reservoirs must be predispensed by some other method such as a 12-channel pipette. For optimization experiments the reservoirs are filled automatically.) Standard screening, additive and microseeding experiments are included as well as microbatch-under-oil screens (also down to 100 + 100 nl). Optimization experiments can be carried out using up to 7 ingredients with full pH control, including multi-variate designs such as the Central Composite. In addition, quick-and-easy 2-D grid optimizations with up to 4 ingredients can be carried out in a few minutes. Oryx4 is the product for those users who do not want to do multi-variate optimization. As with the Oryx8, it performs standard screening, additive, microseeding, sitting drop and hanging drop experiments and microbatch-under-oil screens (also down to 100+100 nl). Oryx4 can be upgraded to Oryx8 if multi-variate optimization becomes a requirement. OryxNano is a compact option which has the capability of the Oryx4, except for hanging drop, microbatch and additive experiments. OryxNano can do quick-and-easy 2-D grid optimizations with up to 4 ingredients. |
Feature | |||
Sitting drop: |
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Single protein screening, multiple drops per reservoir | ✔ | ✔ | ✔ |
Two protein screening | ✔ | ✔ | ✔ |
Three protein screening | ✔ |
| ✔ |
MMS (microseed matrix screening) | ✔ | ✔ | ✔ |
Additive screening |
| ✔ | ✔ |
Additive screening with MMS |
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| ✔ |
Hanging drop: |
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Hanging drop - up to five drops dispensed per cover slide |
| ✔ | ✔ |
Microbatch with automatic oiling: |
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Additive experiments |
| ✔ | ✔ |
Microseed Matrix Screening |
| ✔ | ✔ |
Optimization: |
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Quick-and-easy 2-d grid, 3 ingredients | ✔ | ✔ | ✔ |
Quick-and-easy 2-d grid, 4 ingredients | ✔ |
| ✔ |
7-d Optimization grids |
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| ✔ |
Central composite, multi-variate experimental designs (7-channel) |
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| ✔ |
Rapid reservoir filling for optimization |
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| ✔ |
Tools: |
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Microlytic crystal former filling |
| ✔ | ✔ |
LCP upgrade: |
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Compatible with LCP dispensing arm add-on |
| ✔ | ✔ |
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