ORYX4/8 Specification

Nov 2016

General

Crystallization Methods 1. Microbatch
2. Sitting Drop
3. Hanging Drop
4. Lipidic Cubic Phase
Ambient temperature 4 to 30C
Computer requirement PC with Windows 7, 8 or 10


Vapor Diffusion Method

Volume of droplet 0.1 + 0.1 to 8.0 + 8.0 microliters
Plates accommodated All high quality plates and hanging drop cover slides can be used
Number of wells dispensed 1 to 96x5


Microbatch Method

Volume range of crystallization trial 0.1 + 0.1 to 8.0 + 8.0 microliters
Typical volume of crystallization trial for screening. 0.1 + 0.1 to 0.3 + 0.3 microliters
Typical volume of crystallization trial for optimization 0.6 to 2.0 microliters   (Oryx8 only)
Volume of protein dispensed per trial 0.1 to 5 microliters
Number of crystallization trials set up per run 1 to 384 (or more)
Number of solutions dispensed in each trial for optimization 1 to 7 (including water)
Plates accommodated All high quality plates can be used


Lipidic Cubic Phase

Volume range of Lipidic Cubic Phase 10 nL to 1.0 microliters or larger
Plates accommodated All high quality sandwich or sitting drop plates can be used
Number of wells dispensed 1 to 96x5
Number of solutions dispensed in each trial for optimization 1 to 7 (including water)


Microtips

Number of bores 7, 5, 4, 3 or 2
Cross-section of microtip at tip 0.45 - 0.95 mm
Cross-section of each bore 150 microns I.D.
Dead volume Zero
Material Transparent flexible water repellent fluoropolymer (FEP).  Protein channel is treated to prevent the air-bubble from breaking up.


Universal Syringe Driver

Number of discrete steps for syringe volume More than 22,000
Displacement for each step 3.2 microns
Volume dispensed per step with a 100 microlitre syringe. 5.2 nl
Maximum speed 4.0 mm/second

Accuracy of Universal Syringe Driver
R.M.S. error per step +/- 8%
Nominal maximum error per step 16%
Nominal maximum cumulative error over complete linear displacement 2 steps


Automatic XYZV Plate Loader

Linear displacement of table:
travel, first horizontal axis (X) 321 mm
travel, second horizontal axis (Y) 151 mm
travel, first vertical axis (Z) 50 mm
travel, second vertical axis (V) 52 mm
Nominal maximum cumulative error over complete linear displacement 0.1 mm
Length required on bench 680 mm
Depth required on bench 565 mm
Height in extended position including
z loop
340 mm
Weight 11.8 kg
Time taken to dispense 96 well 1 drop experiment into e.g. SwissCI MRC 2 drop plate <9 min
Maximum speed:
X 75 mm/s
Y 55 mm/s
Z &V 25 mm/s
The speed is software controllable and is fully variable


Chassis

Width 275 mm
Depth 200 mm
Height   450 mm
Weight 15kg - Ory8
8kg - Ory4


No. of Chassis Units

Oryx8 2
Oryx4 1


Each Oryx is controlled by one MCC Motion Control Center.  Click here for MCC specification.

See also Visio File 

2017 Douglas Instruments Ltd