References


New methods and theoretical studies:

Kolek, S. A., Braeuning, B., & Shaw Stewart, P. D
A novel microseeding method for the crystallization of membrane proteins in lipidic cubic phase. Acta F, 2016.
On-line at http://scripts.iucr.org/cgi-bin/paper?ub5090

Shaw Stewart, P., & Mueller-Dieckmann, J.
Automation in biological crystallization. Acta F, 2014.
On-line at http://scripts.iucr.org/cgi-bin/paper?s2053230x14011601

Moraes, I., Evans, G., Sanchez-Weatherby, J., Newstead, S., & Stewart, P. D. S.
Membrane protein structure determination—the next generation, 2014. BBA Biomembranes
On-line at http://www.sciencedirect.com/science/article/pii/S0005273613002447

Abuhammad, A., Lowe, E. D., McDonough, M. A., Shaw Stewart, P. D., Kolek, S. A. , Sim, E., & Garman, E. F.
Structure of arylamine N-acetyltransferase from Mycobacterium tuberculosis determined by cross-seeding with the homologous protein from M. marinum: triumph over adversity. Acta D, 2013.
On-line at http://scripts.iucr.org/cgi-bin/paper_yard?wd5214

Patrick D. Shaw Stewart, Stefan A. Kolek, Richard A. Briggs, Naomi E. Chayen and Peter F.M. Baldock. 'Random Microseeding: A Theoretical and Practical Exploration of Seed Stability and Seeding Techniques for Successful Protein Crystallization'. Cryst. Growth Des., 2011, 11 (8), pp 3432–3441. On-line at http://pubs.acs.org/doi/abs/10.1021/cg2001442.  If you do not have a subscription to Crystal Growth and Design, click here to obtain a free copy.

Jose A. Gavira, M. A. Hernandez-Hernandez, Luis A. Gonzalez-Ramirez, Richard A. Briggs, Stefan A. Kolek, and Patrick D. Shaw Stewart. "Combining Counter-Diffusion and Microseeding to Increase the Success Rate in Protein Crystallization." Cryst. Growth Des., 2011, 11 (6), pp 2122–2126. Available on-line at http://pubs.acs.org/doi/abs/10.1021/cg101380j

Garcia-Caballero, Shaw Stewart, Briggs, Kolek and others. "Optimization of Protein Crystallization: The OptiCryst Project". Cryst. Growth Des., 2011, 11 (6), pp 2112–2121. Available on-line at http://pubs.acs.org/doi/abs/10.1021/cg1013768

Benjamin Stieglitz, Katrin Rittinger, and Lesley F. Haire.  "Crystallization of SHARPIN using an automated two-dimensional grid screen for optimization".  Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 2012 July 1; 68(Pt 7): 816–819.   Available on-line at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388930/

Galina Obmolova,* Thomas J. Malia, Alexey Teplyakov, Raymond Sweet and Gary L. Gilliland. 'Promoting crystallization of antibody–antigen complexes via microseed matrix screening.' Acta Crystallographica Section D 66 (2010) 927–933.  Open-access at http://journals.iucr.org/d/issues/2010/08/00/bw5361/bw5361.pdf

A. G. Villaseñor, A. Wong, A. Shao, A. Garg, A. Kuglstatter and S. F. Harris. 'Acoustic matrix microseeding: improving protein crystal growth with minimal chemical bias.' Acta Crystallographica Section D 66 (2010) 568-576. On-line at http://scripts.iucr.org/cgi-bin/paper?S0907444910005512

Allan D’Arcy, Frederic Villarda, May Marsh. An automated microseed matrix-screening method for protein crystallization.  Acta Crystallographica section D 63 (2007), 550–554.  (This paper describes the use of microseeding in the SCREENING step of crystallization - which has proved to be a very easy and powerful technique.)

Gregory Ireton and Barry Stoddard.  'Microseed matrix screening to improve crystals of yeast cytosine deaminase'.  Acta Crystallographica section D60 (2004) 601–605.  Available on-line at http://scripts.iucr.org/cgi-bin/paper?S0907444903029664

G. B. Mortuza, L.F. Haire, A. Stevens, S.J. Smerdon, J.P. Stoye, I.A. Taylor. High-resolution structure of a retroviral capsid hexameric amino-terminal domain.  Nature 431 (2004), pp 481-485. (This paper describes the use of the vapor batch plate with isopropanol.)

A. D'Arcy, A. Mac Sweeney, M. Stihle, A. Haber.  The advantages of using a modified microbatch method for rapid screening of protein crystallization conditions.  Acta Crystallographica section D 59 (2003), pp 396-399.

P.F.M. Baldock, P.D. Shaw Stewart.  Practical experimental design techniques for automatic and manual protein crystallization. Journal of Crystal Growth 196 (1999) 665 - 673

 N.E. Chayen. Comparative studies of protein crystallization by vapor diffusion and microbatch. Acta Cryst. D54 (1998), pp 8 - 15. (This paper describes converting microbatch conditions to vapor diffusion, and also seems to be the first publication that mentions crystallization in less than 1 microlitre drops.)

P.F.M. Baldock, V. Mills, P.D. Shaw Stewart. A comparison of microbatch and vapour diffusion for initial screening of crystallization conditions. Journal of Crystal Growth. 168 (1996), pp 170-174.

A.D'Arcy, C.Elmore, M.Stihle, J.E.Johnston.  A novel approach to crystallising proteins under oil. Journal of Crystal Growth 168 (1996) 175-180.

P.D. Shaw Stewart, M. Khimasia. Predispensed Gradient Matrices - a New Rapid Method of Finding Crystallization Conditions. Acta Crystallographica. D 50 (1994), pp 441-442.

E.E.G. Saridakis, P.D. Shaw Stewart, L.F. Lloyd, D.M. Blow. Phase Diagram and Dilution Experiments in the Crystallization of Carboxypeptidase G2. Acta Crystallographica. D 50 (1994), pp 293-297.

D.M. Blow, N.E. Chayen, L.F. Lloyd, E. Saridakis. Control of Nucleation of Protein Crystals. Protein Science. 3 (1994), pp 1638-1643.

N.E. Chayen, J. Radcliffe, D.M. Blow. The Control of Nucleation in the Crystallization of Lysozyme. Protein Science. 2 (1993), pp 113-118.

L.F.Lloyd, P.Brick, D.M.Blow. Crystallization studies on HIV-1 reverse transcriptase. Journal of Crystal Growth. 122 (1992), pp 355-359.


Description of microbatch, IMPAX (the predecessor of Oryx) and Oryx:

P. D. Shaw Stewart. The development and applications of a new liquid handling device. M. Phil. Thesis, Imperial College of Science and Technology, May-1988. Available from Imperial College Library on application. https://spiral.imperial.ac.uk/handle/10044/1/1227/browse?type=dateissued

N.E. Chayen, P.D. Shaw Stewart, D.L. Maeder, and D.M. Blow. An Automated System for Micro-batch Protein Crystallization and Screening. Journal of Applied Crystallography. 23 (1990), pp 297-302.

N.E. Chayen, P.D. Shaw Stewart, D.M. Blow. Microbatch Crystallization Under Oil - a New Technique Allowing Many Small-volume Crystallization Trials. Journal of Crystal Growth. 122 (1992), pp 176-180.

N.E. Chayen, P.D. Shaw Stewart, P. Baldock. New Developments of the IMPAX Small-Volume Automated Crystallization System. Acta Crystallographica. D 50 (1994), pp 456-458.

A.K. Shah, Zhi-Jie Liu, P.D. Shaw Stewart, F.D. Schubot, J.P. Rose, M.G. Newton and B-C. Wang. On increasing protein-crystallization throughput for X-ray diffraction studies.  Acta Crystallographica D 61 (2005), pp 123-129


Successful crystallizations using microbatch and Oryx:

S. Yamada, Y. Suzuki, T. Suzuki, M. Q. Le, C. A. Nidom, Y. Sakai-Tagawa, Y. Muramoto, M. Ito, M. Kiso, T. Horimoto, K. Shinya, T. Sawada, M. Kiso, T. Usui, T. Murata, Y. Lin, A. Hay, L. F. Haire, D. J. Stevens, R. J. Russell, S. J. Gamblin, J. J. Skehel & Y. Kawaoka. Haemagglutinin mutations responsible for the binding of H5N1 influenza A viruses to human-type receptors.  Nature (444) 16 November 2006 doi:10.1038/nature05264

R. J. Russell, L. F. Haire, D. J. Stevens, P. J. Collins, Y. P. Lin, G. M. Blackburn, A. J. Hay, S. J. Gamblin & J. J. Skehel. The structure of H5N1 avian influenza neuraminidase suggests new opportunities for drug design.  Nature, (443) Sept 7, 2006 45-49 (N1 neuraminidase structure solved with only 0.6 mg of protein using Oryx8).

G. B. Mortuza, L.F. Haire, A. Stevens, S.J. Smerdon, J.P. Stoye, I.A. Taylor. High-resolution structure of a retroviral capsid hexameric amino-terminal domain.  Nature 431 (2004), pp 481-485.

Y. Korkhin, F. Frolow, O. Bogin, M. Peretz, A.J. Kalb, Y. Burstein. Crystalline alcohol dehydrogenases from the mesophilic bacterium Clostridium beijerinckii and the thermophilic bacterium Thermoanaerobium brockii: preparation, characterization and molecular symmetry. Acta Crystallographica. D 52 (1996), pp 882-886.

N.E. Chayen, E. Conti, C. Vieille, J.G. Zeikus. Crystallization and initial x-ray analysis of xylose isomerase from Thermotoga neapolitana. Acta Crystallographica. D 53 (1997), 2, pp 229-230.

F. Sica, S. Adinolfi, L. Vitagliano, A. Zagari, S. Capasso, L. Mazzarella. Cosolute effect on crystallization of two dinucleotide complexes of bovine seminal ribonuclease from concentrated salt solutions. Journal of Crystal Growth. 168 (1996), 1-4, pp 192-197.

Y. Korkhin, F. Frolow, O. Bogin, M. Peretz, A.J. Kalb (Gilboa), Y. Burstein. Crystalline alcohol dehydrogenases from the mesophilic bacterium Clostridium beijerinckii and the thermophilic bacterium Thermoanaerobium brockii: preparation, characterization and molecular symmetry. Acta Crystallographica. D 52 (1996), pp 882-886.

S.W. Cowan-Jacob, J. Rahuel, A. Nagai, G. Iwasaki, D. Ohta. Crystallization and preliminary crystallogrphic analysis of cabbage histidinol dehydrogenase. Acta Crystallographica. D 52 (1996), 6, pp 1188-1190.

E. Conti, L.F. Lloyd, J. Atkins, N.P. Franks, P. Brick. Crystallization and preliminary diffraction studies of firefly luciferase from Photinus pyralis. Acta Crystallographica. D 52 (1996), 4, pp 876-878.

N.E. Chayen, E.J. Gordon, S.E.V. Phillips, E.E.G. Saridakis, P.F. Zagalsky. Crystallization and initial x-ray analysis of beta-crustacyanin, the dimer of apoproteins A(2) and C-1, each with a bound astaxanthin molecule. Acta Crystallographica. D 52 (1996), pp 409-410.

B.P. O’Hara, A.M. Hemmings, D.J. Buttle, L.H. Pearl. Crystal-structure of glycyl endopeptidase from carcia-papaya - a cysteine endopeptidase of unusual substrate-specificity. Biochemistry. 34 (1995), 40, 13190-13195.

R. Savva, L.H. Pearl. Cloning and expression of the uracil-DNA glycosylase inhibitor (UGI) from bacteriophage-PBS-1 and crystallization of a uracil-DNA glycosylase-UGI complex. Proteins-Structure Function and Genetics. 22 (1995), 3, pp 287-289.

L.F. Lloyd, P. Brick, L. Mei-Zhen, N.E. Chayen, D.M.Blow. Many crystal forms of human immunodeficiency virus reverse transcriptase. Journal of Molecular Biology. 217 (1991), pp 19-22.

S.A. Wilson, N.E. Chayen, A.M. Hemmings, R.E. Drew, L.H. Pearl. Crystallization of and Preliminary X-ray Data for the Negative Regulator (AmiC) of the Amidase Operon of Pseudomonas-aeruginosa. Journal of Molecular Biology. 222 (1991), 4, pp 869-871.

 


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