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How to get the right number of crystals for data collection and soaking experiments: microseeding optimization

Microseeding is a very powerful method of accurately controlling the number of crystals in a drop. Often when microseeding is used for screening (random MMS microseeding) or optimization showers of small crystals form. To control the number of crystals you can set up dilutions of the seed stock. However, you can end up doing many rounds of seeding - how can you find the best dilution as quickly as possible?

Oryx robots have scripts to test dilutions of the seed stock systematically to find the best concentration:

The 'Cross Matrix' experiment can test up to 12 seed stocks against 8 precipitants.

The 'Additive Scatter' experiment can test up to 5 seed stocks against a 2D gradient, e.g. pH vs precipitant.

Case Study using the 'Cross Matrix' experiment:


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Douglas Instruments will be at the following meetings:

Visit our booth and pick up a microseeding toolkit containing everything you need to do a MMS Microseeding experiment including the Hampton seed bead and crystal crusher.

AsCA 2015 Kolkata

December 5th - 8th.

BCA 2016 Nottingham

April 4th - 7th.

FEBS Advanced methods in macromolecular crystallization, Nove Hrady

June 27th - July 2nd.

ICCBM 2016 Prague

July 2nd - 7th.

Recent citations of Douglas Instruments products

Purification and Bicelle Crystallization for Structure Determination of the E. coli Outer Membrane Protein TamA

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Methods in Molecular Biology 1329 (2014): 259-270

Structure of the novel monomeric glyoxalase I from Zea mays

G. L. Turra, R. B. Agostini, C. M. Fauguel, D. A. Presello, C. S. Andreo, J. M. González and V. A. Campos-Bermudez

Acta Cryst D 71.10 (2015): 2009-2020

Discovery and characterization of natural tropolones as inhibitors of the antibacterial target CapF from Staphylococcus aureus

Koichiro Nakano, Takeru Chigira, Takamitsu Miyafusa, Satoru Nagatoishi, Jose M. M. Caaveiro & Kouhei Tsumoto

Scientific Reports 5 (2015): 15337



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Berkshire RG17 7HD
United Kingdom

Tel: + (44) 1488 649090
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