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Protein crystallization tips and tricks - analysis using polarizers with the UVXPO 'MRC' 2-drop plate

The new UVXPO 'MRC' 2 drop plate from Swissci offers advances in UV and cross-polarized imaging. The new plate has exactly the same dimensions as the original MRC 2-drop crystallization plate, but is made from a special polymer that is designed to maximize UV and polarized light.

Key features of the UVXPO 'MRC' 2-drop plate:

        -  Uniform background when using polarized light.
        -  UV imager compatible.
        -  More hydrophilic surface than the original MRC 2 drop UV plate (COC) gives better dispensing.
        -  OryxNano, Oryx4 and Oryx8 can dispense screening and optimization experiments.
        -  Oryx8 can dispense reservoir solutions for optimization.
        -  Oryx LCP systems can dispense LCP experiments to the plate.

The UVXPO 2-drop plate is available from Douglas Instruments.

Tips and tricks for using the UVXPO plate:

Although the UVXPO MRC plate has a very low uniform birefringence, when the plate is sealed, often the seal does have birefringence and acts a wave plate. This means one is unable to get a true black or white background as the polarizer is rotated with a normal plate seal.

To cancel out the waveplate effect, simply add a second layer of the same plate seal exactly perpendicular to the first.


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For anything else please contact

Douglas Instruments will be at the following meetings:

Visit our booth and pick up a microseeding toolkit containing everything you need to do a MMS Microseeding experiment including the Hampton seed bead and crystal crusher.

Dilemmas in structural biology, NHRF, Athens

14 – 17 February 2016

BCA 2016 Nottingham

April 4th - 7th.

Crystallization: focus on micro and nano crystals and high throughput methods, 2016 at SLAC

April 19th - 22nd.

FEBS Advanced methods in macromolecular crystallization, Nove Hrady

June 27th - July 2nd.

ICCBM 2016 Prague

July 2nd - 7th.

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United Kingdom

Tel: + (44) 1488 649090
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