View web version
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www.douglas.co.uk
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Set up Sitting Drop and Microbatch-Under-Oil experiments at the same time
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Available at the press of one button!
- Two screening methods
- Up to 4 drop ratios
- Additives (e.g. seed stock)
Video of Combined Microbatch and Vapor Diffusion experiment
Video of Microbatch-Under-Oil Optimization Gradient experiment
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Visit our booth at ACA, Denver for more information.
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- 20 µL of protein is enough for both methods (2x 96x 100+100 nL drops)
- Automatic oil dispensing onto aqueous drop. This give much more consistent drop size, shape and mixing
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Advantages of the microbatch under oil technique include:
- Microbatch reduces skins on the drop surface.
- Microbatch conditions are easier to scale up.
- The rate of evaporation can be controlled by oil. (Paraffin gives slow evaporation, silicone gives rapid evaporation.)
For optimization under oil pure paraffin oil can be used, which reduces evaporation to a minimum
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Douglas Instruments Vapour Batch plates are available in Hydrophillic or Hydrophobic material. Hydrophillic are often the best option for screening.
Hydrophobic are useful for some optimization experiments and low surface tension solutions.
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To request a quote or demonstration please contact Hilary@douglas.co.uk.
For any technical or product support questions please contact Stefan@douglas.co.uk
For anything else please contact Info@douglas.co.uk
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Douglas Instruments will be at the following meetings:
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Visit our booth and pick up a Microseeding Tool Kit containing
everything you need to do a MMS microseeding experiment. The kit includes:
- Hampton Research Seed Bead
- Hampton Research Crystal Crusher
- Instructions and advice for making a seed stock, and setting up an MMS experiment.
- References to papers about MMS microseeding and 'cross-seeding'.
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Recent mentions of Douglas Instruments products
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Crystal Structure of Marburg Virus VP40 Reveals a Broad, Basic Patch for Matrix Assembly and a Requirement of the N-Terminal Domain for Immunosuppression
Oda, Shun-ichiro, Takeshi Noda, Kaveesha J. Wijesinghe, Peter Halfmann, Zachary A. Bornholdt, Dafna M. Abelson, Tammy Armbrust, Robert V. Stahelin, Yoshihiro Kawaoka, and Erica Ollmann Saphire
Journal of virology 90.4 1839-1848 (2016)
Structural determinants of reductive terpene cyclization in iridoid biosynthesis
Hajo Kries, Lorenzo Caputi, Clare E M Stevenson, Mohammed O Kamileen, Nathaniel H Sherden, Fernando Geu-Flores, David M Lawson & Sarah E O'Connor
Nature chemical biology, 12(1), pp.6-8. (2016)
Crystal structure of Cdc11, a septin subunit from Saccharomyces cerevisiae
Anton Brausemann, Stefan Gerhardt, Anne-Kathrin Schott, Oliver Einsle, Andreas Große-Berkenbusch, Nils Johnsson, Thomas Gronemeyer
Journal of structural biology (2016)
The molecular characterizations of surface proteins hemagglutinin and neuraminidase from recent H5Nx avian influenza viruses
Hua Yang, Paul J. Carney, Vasiliy P. Mishin, Zhu Guo, Jessie C. Chang, David E. Wentworth, Larisa V. Gubareva and James Stevens
Journal of Virology. JVI-00180 (2016)
Structure-Function Relationships in L-Amino Acid Deaminase, a Flavoprotein Belonging to a Novel Class of Biotechnologically Relevant Enzymes
Paolo Motta, Gianluca Molla, Loredano Pollegioni and Marco Nardini
Journal of Biological Chemistry, pp.jbc-M115. 2016
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Douglas Instruments Ltd. All rights reserved.
Douglas Instruments Ltd
East Garston
Hungerford
Berkshire RG17 7HD
United Kingdom
Tel: + (44) 1488 649090
US toll free: 1-877-225-2034
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Do you
have a comment or question about protein crystallization or robotics that
members of our bulletin board may be able to help you with?
Subscribe
to the Automatic Protein Crystallization Group
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Visit this group
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Douglas Instruments
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