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27/10/2017

DIXC screen for Cryo-EM

A new screen for Cryo-EM

Douglas Instruments has designed a screen that aims to improve the behaviour of macromolecules for cryoEM experiments. The screen includes ions at both extremes of the Hofmeister series (i.e. both chaotropes and kosmotropes), organic and inorganic buffers, detergents, sugars and alcohols. In order to reduce the noise generated by the ingredients of the screen in cryoEM images, low molecular weight compounds based on elements near the top of the periodic table were chosen where possible.

DIXC Conditions:

Well

Conc

Chemical

pH

1 (A1)

1 M

Mg chloride

2 (A2)

1 M

Li chloride

3 (A3)

1 M

Na chloride

4 (A4)

1 M

K chloride

5 (A5)

1 M

NH4 chloride

6 (A6)

1 M

NH4 sulfate

7 (A7)

1 M

Li nitrate

8 (A8)

1 M

Na formate

4

9 (A9)

1 M

Na citrate

10 (A10)

1 M

Na tartrate

11 (A11)

1 M

Na, K phosphate

7

12 (A12)

1 M

Na, K phosphate

4.5

13 (B1)

1 M

K thiocyanate

14 (B2)

1 M

Urea

15 (B3)

1 M

Guanidinium chloride

16 (B4)

1 M

Na malate

3.5

17 (B5)

1 M

Na acetate

5

18 (B6)

1 M

Na succinate

5.5

19 (B7)

1 M

Na maleate

6

20 (B8)

1 M

Na carbonate

6.5

21 (B9)

1 M

TEA

7.5

22 (B10)

1 M

Tris

8

23 (B11)

1 M

Bicine

8.5

24 (B12)

1 M

Na borate

9

25 (C1)

0.5 M

Glucose

26 (C2)

0.5 M

Fructose

27 (C3)

0.5 M

Galactose

28 (C4)

0.5 CMC

Fluorinated octyl maltoside

29 (C5)

0.5 CMC

Tetraethylene Glycol Monooctyl Ether (T350)

30 (C6)

0.5 CMC

Pentaethylene glycol monododecyl ether

31 (C7)

0.5 CMC

N-Decanoyl-N-methylglucamine

32 (C8)

0.5 CMC

n-Dodecyl -D-maltoside

33 (C9)

50 %

MPD

34 (C10)

50 %

Methanol

35 (C11)

50 %

Enthanol

36 (C12)

50 %

Isopropanol

37 (D1)

50 %

Glycerol

38 (D2)

50 %

Ethylene glycol

39 (D3)

0.1 %

Ultra-low melting point agarose

40 (D4)

1 M

Resorcinol

7

41 (D5)

1 M

Gly-Gly-Gly

7

42 (D6)

1 M

Oxamic acid

7

43 (D7)

1 M

Barbituric acid

7

44 (D8)

1 M

Na Malonate

7

45 (D9)

1 M

Glycerol 2 phosphate

7

46 (D10)

100 %

User defined

47 (D11)

100 %

User defined

48 (D12)

100 %

Water

 

 

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