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Explore chemical space for cryoEM and crystallizationThe Oryx8 in combination with the SpectroLight 600 can identify promising conditions for structure determination by cryoEM (and crystallization). For cryoEM, screens such as the DIXC screen comprising of detergents, ions from the extremes of the Hofmeister series, buffers and substrates can be used. Favorable conditions can then be optimized before setting up cryoEM grids.
Screening and optimization experiments (with cryoEM and/or crystallography in mind) can be dispensed by the Oryx8, then efficiently analyzed by the SpectroLight600. Several hundred samples can be analyzed per day, before choosing the best to pass on for analysis by cryoEM. Representative results for a membrane protein in a 96 well detergent screen:
DIXC screen for Cryo-EM
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| Well | Conc | Chemical | pH |
| 1 (A1) | 1 M | Mg chloride |
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| 2 (A2) | 1 M | Li chloride |
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| 3 (A3) | 1 M | Na chloride |
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| 4 (A4) | 1 M | K chloride |
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| 5 (A5) | 1 M | NH4 chloride |
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| 6 (A6) | 1 M | NH4 sulfate |
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| 7 (A7) | 1 M | Li nitrate |
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| 8 (A8) | 1 M | Na formate | 4 |
| 9 (A9) | 1 M | Na citrate |
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| 10 (A10) | 1 M | Na tartrate |
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| 11 (A11) | 1 M | Na, K phosphate | 7 |
| 12 (A12) | 1 M | Na, K phosphate | 4.5 |
| 13 (B1) | 1 M | K thiocyanate |
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| 14 (B2) | 1 M | Urea |
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| 15 (B3) | 1 M | Guanidinium chloride |
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| 16 (B4) | 1 M | Na malate | 3.5 |
| 17 (B5) | 1 M | Na acetate | 5 |
| 18 (B6) | 1 M | Na succinate | 5.5 |
| 19 (B7) | 1 M | Na maleate | 6 |
| 20 (B8) | 1 M | Na carbonate | 6.5 |
| 21 (B9) | 1 M | TEA | 7.5 |
| 22 (B10) | 1 M | Tris | 8 |
| 23 (B11) | 1 M | Bicine | 8.5 |
| 24 (B12) | 1 M | Na borate | 9 |
| 25 (C1) | 0.5 M | Glucose |
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| 26 (C2) | 0.5 M | Fructose |
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| 27 (C3) | 0.5 M | Galactose |
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| 28 (C4) | 0.5 CMC | Fluorinated octyl maltoside |
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| 29 (C5) | 0.5 CMC | Tetraethylene Glycol Monooctyl Ether (T350) |
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| 30 (C6) | 0.5 CMC | Pentaethylene glycol monododecyl ether |
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| 31 (C7) | 0.5 CMC | N-Decanoyl-N-methylglucamine |
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| 32 (C8) | 0.5 CMC | n-Dodecyl ß-D-maltoside |
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| 33 (C9) | 50 % | MPD |
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| 34 (C10) | 50 % | Methanol |
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| 35 (C11) | 50 % | Enthanol |
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| 36 (C12) | 50 % | Isopropanol |
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| 37 (D1) | 50 % | Glycerol |
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| 38 (D2) | 50 % | Ethylene glycol |
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| 39 (D3) | 0.1 % | Ultra-low melting point agarose |
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| 40 (D4) | 1 M | Resorcinol | 7 |
| 41 (D5) | 1 M | Gly-Gly-Gly | 7 |
| 42 (D6) | 1 M | Oxamic acid | 7 |
| 43 (D7) | 1 M | Barbituric acid | 7 |
| 44 (D8) | 1 M | Na Malonate | 7 |
| 45 (D9) | 1 M | Glycerol 2 phosphate | 7 |
| 46 (D10) | 100 % | User defined |
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| 47 (D11) | 100 % | User defined |
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| 48 (D12) | 100 % | Water |
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