Measuring Protein Aggregation and Stability Using High-Throughput Biophysical Approaches
Kwan, T.O., Kolek, S.A., Danson, A.E., Reis, R.I., Camacho, I.S., Stewart, P.D.S. and Moraes, I.
Frontiers in Molecular Biosciences, p.481 (2022)
A novel microseeding method for the crystallization of membrane proteins in lipidic cubic phase
Kolek, S. A., Braeuning, B., & Shaw Stewart, P. D
Acta Crystallographica Section F: Structural Biology Communications 72.4 (2016): 307-312
Automation in biological crystallization
Shaw Stewart, P.D, & Mueller-Dieckmann, J.
Acta Crystallographica Section F: Structural Biology Communications 70.6 (2014): 686-696
Membrane protein structure determination — the next generation
Moraes, I., Evans, G., Sanchez-Weatherby, J., Newstead, S., & Shaw Stewart, P. D.
Biochimica et Biophysica Acta (BBA)-Biomembranes 1838.1 (2014): 78-87
Structure of arylamine N-acetyltransferase from Mycobacterium tuberculosis determined by cross-seeding with the homologous protein from M. marinum: triumph over adversity
Abuhammad, A., Lowe, E. D., McDonough, M. A., Shaw Stewart, P. D., Kolek, S. A.
, Sim, E., & Garman, E. F.
Acta Crystallographica Section D: Biological Crystallography 69.8 (2013): 1433-1446
Random microseeding: a theoretical and practical exploration of seed stability and seeding techniques for successful protein crystallization
Shaw Stewart, P. D., Kolek, S. A., Briggs, R. A., Chayen, N. E., & Baldock, P. F.
Crystal Growth & Design 11.8 (2011): 3432-3441
Full list of references
A list of publications describing Oryx, theoretical studies using Oryx and
New methods. This list includes publications by Douglas Instruments
Sample preparation for frontier structural biology
Microseed Matrix Screening with Lipidic Cubic Phase
Microbatch-under-oil for XFEL
Advanced Microseeding including LCP
Robotic LCP dispensing
Coupling counterdiffusion and micro-seeding techniques to increase
protein crystallization succeed
Articles and Research Reports
Further data mining of the PDB includes the concentration of protein, the
temperature, and the pH used in crystallization experiments.
Research report 10, September 2011
Microseed Matrix Screening (MMS).
Research report 9, August 2007
Data mining of the PDB reveals the most successful organic and salt
Research report 8, August 2006
Screening: a summary of studies comparing microbatch with vapor
Research report 7, June 2003
Membrane proteins crystallized using microbatch. We know of four so far.
Research report 6, March 2001
Analysis of open reading frames from Pyrococcus furiosus
Research report 5, June 1999
Practical Experimental Design Techniques for Automatic and Manual
(published in the Journal of Crystal Growth 196 (1999) 665 - 673)
Research report 4, August 1998
A Comparison of Microbatch and Vapor Diffusion for Initial Screening of Crystallization
Conditions (published in the Journal of Crystal Growth 168 (1996) 170-174)
Research report 3, November 1995
Harvesting Crystals from Microbatch for Cryocrystallography
Research report 2, October 1995
Crystallization of a protein by microseeding after establishing its phase
Research report 1, August 1995