Information about random Matrix Microseeding (rMMS)

• Introduction to rMMS
• Instructions for making Seed Stock
• Making Seed Stock from MB under oil (PDF)
• Help with rMMS - online questionnaire

Information about Under Oil Crystallization

• Introduction to Microbatch Under Oil
• Microbatch Experiments with Oryx8
• Plates for Under Oil Crystallization
• Harvesting from isopropanol Under Oil

Establish the phase diagram for your protein

• The Phase Diagram experiment

Systematically test seed stock dilution, additive ingredients or multiple protein samples

• 'Cross Matrix' Optimization with Oryx 8
• Microseed dilution optimization
• 'Reshuffling' optimization

Information about LCP dispensing with Oryx

• Preparing LCP with our mixer (PDF)
• LCP screening instructions (PDF)
• rMMS with LCP: method in Acta F

A few simple guidelines for scaling up from nano-drops to micro-drops

• Scaling Up

Three simple rules of thumb allow users to convert vapor diffusion crystallization conditions to microbatch

• VD to MB: three simple rules
• Hampton Research calculator for VD-MB conversion (xlsx)

Optimization Methods with Oryx

• Rational experimental design techniques
• Optimization with Oryx8
• Optimization for XFEL poster (PDF)
• Instructions: Optimization with Oryx8 (PDF)

Dispensing to 24 well Cover Slides with Oryx

• 24-Well Hanging drop: Oryx4 and 8
• Hanging drop experiments

Tips and tricks for crystallization plates

• Cross-polarization with sitting drop plates

Simple step-by-step instructions for users covering maintenance and experiments

• Cards for Oryx users

Consumable supplies for Oryx robots

• Consumable supplies

DLS experiments for Cryo-EM

• DLS screening for Cryo-EM
• DIXC screen

The 'Good Dispensing Guide' for Oryx robots

• The 'Good Dispensing Guide'

Specifications for Oryx robots

• Oryx8 and Oryx4 specifications
• OryxNano specifications
• MCC controller specifications
• Information for new users