See also:

  • New user information and software requirements
  • Oryx8 and Oryx4 specifications
  • OryxNano specifications
  • MCC controller specifications
  • ORYX4/8 Specification

    General

    Crystallization Methods 1. Microbatch
    2. Sitting Drop
    3. Hanging Drop
    4. Lipidic Cubic Phase
    Ambient temperature 4 to 30°C
    Computer requirement PC with Windows 11, 10, 8 or 7


    Vapor Diffusion Method

    Volume of droplet 0.1 + 0.1 to 8.0 + 8.0 microliters
    Plates accommodated All high quality plates and hanging drop cover slides can be used
    Number of wells dispensed 1 to 96x5


    Microbatch Method

    Volume range of crystallization trial 0.1 + 0.1 to 8.0 + 8.0 microliters
    Typical volume of crystallization trial for screening. 0.1 + 0.1 to 0.3 + 0.3 microliters
    Typical volume of crystallization trial for optimization 0.6 to 2.0 microliters   (Oryx8 only)
    Volume of protein dispensed per trial 0.1 to 5 microliters
    Number of crystallization trials set up per run 1 to 384 (or more)
    Number of solutions dispensed in each trial for optimization 1 to 7 (including water)
    Plates accommodated All high quality plates can be used


    Lipidic Cubic Phase

    Volume range of Lipidic Cubic Phase 10 nL to 1.0 microliters or larger
    Plates accommodated All high quality sandwich or sitting drop plates can be used
    Number of wells dispensed 1 to 96x5
    Number of solutions dispensed in each trial for optimization 1 to 7 (including water)


    Microtips

    Number of bores, Oryx8: 7, 4, 3 or 2
    Number of bores, Oryx4: 3 or 2
    Cross-section of microtip at tip 0.45 - 0.95 mm
    Cross-section of each bore 150 microns I.D.
    Dead volume Zero
    Material Transparent flexible water repellent fluoropolymer (FEP).  Protein channel is treated to prevent the air-bubble from breaking up.


    Liquid Handling Accuracy

    The CV (coefficient of variance) has been measured using 50% PEG 4000 and 95% PEG 600 (with 5% red dye) as follows.

    CV for 1 µl total drop volume 3%
    CV for 200 nl total drop volume 5%
    CV for 100 nl total drop volume 6.5%


    Universal Syringe Driver

    Number of discrete steps for syringe volume More than 22,000
    Displacement for each step 3.2 microns
    Volume dispensed per step with a 100 microlitre syringe. 5.2 nl
    Maximum speed 4.0 mm/second

    Accuracy of Universal Syringe Driver
    R.M.S. error per step +/- 8%
    Nominal maximum error per step 16%
    Nominal maximum cumulative error over complete linear displacement 2 steps


    Automatic XYZV Plate Loader

    Linear displacement of table:
    travel, first horizontal axis (X) 321 mm
    travel, second horizontal axis (Y) 151 mm
    travel, first vertical axis (Z) 50 mm
    travel, second vertical axis (V) 52 mm
    Nominal maximum cumulative error over complete linear displacement 0.1 mm
    Length required on bench 680 mm
    Depth required on bench 565 mm
    Height in extended position including
    z loop
    340 mm
    Weight 11.8 kg
    Time taken to dispense 96 well 1 drop experiment into e.g. SwissCI MRC 2 drop plate <9 min
    Maximum speed:
    X 75 mm/s
    Y 55 mm/s
    Z &V 25 mm/s
    The speed is software controllable and is fully variable


    Chassis

    Width 275 mm
    Depth 200 mm
    Height   450 mm
    Weight 15kg - Oryx8
    8kg - Oryx4


    No. of Chassis Units

    Oryx8 2
    Oryx4 1


    Oryx8 Minimum space required

    Minimum space required for Oryx8 in standard configuration.
    (Other configurations are possible)


    Oryx4 Minimum space required

    Minimum space required for Oryx4 in standard configuration.
    (Other configurations are possible)


    Each Oryx is controlled by one MCC Motion Control Center.  Click here for MCC specification.

    See also Visio File 




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